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Proteintech
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Abcam
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Danaher Inc
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Santa Cruz Biotechnology
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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma
doi: 10.1111/jcmm.14792
Figure Lengend Snippet: CD9 is the candidate regulator in glaucoma and overexpression of CD9 maintained TM cell identity. A, Real‐time PCR result of CD9 mRNA in iHTM and GTM 3 cells. n = 6. (B‐C) Protein expression of CD9 in iHTM and GTM 3 cells. n = 6. D, Real‐time PCR result of CD9 mRNA in GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 6. (E‐F) Protein expression of CD9 in iHTM, GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 6. * P < 0.05 vs iHTM group, # P < .05 vs GTM 3 group. G, Immunostaining of Vimentin and Tubulin in iHTM, GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups
Article Snippet: After the membranes were incubated with 5% skim milk at room temperature for 1 hour,
Techniques: Over Expression, Real-time Polymerase Chain Reaction, Expressing, Immunostaining
Journal: Journal of Cellular and Molecular Medicine
Article Title: Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma
doi: 10.1111/jcmm.14792
Figure Lengend Snippet: Knock‐down of CD9 changed TM cell identity. A, Real‐time PCR result of CD9 mRNA in iHTM, iHTM + siRNA, iHTM + si‐NC and GTM 3 groups. n = 6. (B‐C) Protein expression of CD9 in iHTM, iHTM + siRNA, iHTM + si‐NC and GTM 3 groups. n = 6. * P < .05 vs iHTM group, # P < .05 vs iHTM + siRNA group. D, Immunostaining of Vimentin and Tubulin in iHTM, iHTM + siRNA, iHTM + si‐NC and GTM 3 groups
Article Snippet: After the membranes were incubated with 5% skim milk at room temperature for 1 hour,
Techniques: Real-time Polymerase Chain Reaction, Expressing, Immunostaining
Journal: Journal of Cellular and Molecular Medicine
Article Title: Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma
doi: 10.1111/jcmm.14792
Figure Lengend Snippet: CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma. (A,B) Protein expression of ITGA4 in GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 6. (A,C) Protein expression of p‐PI3K in GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 6. (A,D) Protein expression of p‐Akt in GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 6. (A,E) Protein expression of clevage caspase3 in GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 6. * P < .05 vs iHTM group, # P < .05 vs GTM 3 group. (F‐G) FCAS results of cells from iHTM, GTM 3 , GTM 3 + CD9 and GTM 3 + pcDNA3.1 groups. n = 3. * P < .05 vs iHTM group, # P < .05 vs GTM 3 group
Article Snippet: After the membranes were incubated with 5% skim milk at room temperature for 1 hour,
Techniques: Expressing
Journal: Journal of Cellular and Molecular Medicine
Article Title: Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma
doi: 10.1111/jcmm.14792
Figure Lengend Snippet: CD9/ITGA4/PI3K‐Akt axis participating in regulating glaucoma. A, Protein expression of ITGA4, PI3K, Akt and caspase‐3 in iHTM, iHTM + siRNA, iHTM + si‐NC and GTM 3 groups. B, Protein expression of ITGA4, PI3K, Akt and caspase‐3 in GTM 3 + CD9, GTM 3 + CD9+si‐ITGA4 and GTM 3 + CD9+si‐NC groups. C, Mimic mechanism pathway of CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma
Article Snippet: After the membranes were incubated with 5% skim milk at room temperature for 1 hour,
Techniques: Expressing
Journal: International Journal of Molecular Sciences
Article Title: Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration
doi: 10.3390/ijms25020934
Figure Lengend Snippet: Characterization of sEVs released by ARPE-19 cells. Nanoparticle tracking analysis and TEM were used to analyze the size distribution, morphology and to quantify sEVs in ARPE-19 cells under both control conditions ( A ) and high glucose treatment ( B ). Scale bar 200 nm. Difference between number of control sEVs and 35 mM glucose sEVs is presented in particles/mL ( C ). Western blot analysis shows CD9, CD63 and β-Actin expression in sEVs, lysate and cell supernatant ( D ). Values are presented as mean ± SEM ( n = 5). p -value was determined using two-tailed t-test * p < 0.05.
Article Snippet: After blocking with 5% skim milk, membranes were incubated overnight with
Techniques: Western Blot, Expressing, Two Tailed Test